Purification and partial characterization of an alkaline lipase from Pseudomonas pseudoalcaligenes F-111.

نویسندگان

  • S F Lin
  • C M Chiou
  • C M Yeh
  • Y C Tsai
چکیده

An extracellular alkaline lipase of alkalophilic Pseudomonas pseudoalcaligenes F-111 was purified to homogeneity. The apparent molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 32,000, and the isoelectric point was 7.3. With p-nitrophenyl esters as its substrates, the enzyme shows preference for C12 acyl and C14 acyl groups. It was stable in the pH range of 6 to 10, which coincides with the optimum pH range.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 62 3  شماره 

صفحات  -

تاریخ انتشار 1996